Journal: Virulence

Article Title: MiR-140 inhibits classical swine fever virus replication by targeting Rab25 in swine umbilical vein endothelial cells

doi: 10.1080/21505594.2020.1735051

Figure Lengend Snippet: Sequences of primers, miRNAs, and siRNAs used in this study.

Article Snippet: The membrane was blocked with the polyclonal anti-Rab25 antibody (CSB-PA019175LA01HU, cusabio), and monoclonal anti-β-actin antibody (AB0035, Abways).

Techniques: Sequencing, Over Expression, Knockdown

Journal: British Journal of Cancer

Article Title: Androgen-related expression of G-proteins in ovarian cancer

doi: 10.1038/sj.bjc.6605153

Figure Lengend Snippet: Changes in expression of G-proteins after 8-h stimulation by 10 n M DHT

Article Snippet: Monoclonal mouse anti-human Rab25 antibody was used at a concentration of 1 : 500 (clone 3F12F3, ProMab Biotechnologies, Richmond, CA, USA).

Techniques: Expressing

Journal: British Journal of Cancer

Article Title: Androgen-related expression of G-proteins in ovarian cancer

doi: 10.1038/sj.bjc.6605153

Figure Lengend Snippet: Rab25, Rab35 and AR expression in clinical samples. Rab25, Rab35 and AR protein expression levels were determined using immunohistochemical techniques for 154 epithelial cancer samples arrayed on a TMA. Representative sections are shown demonstrating predominantly cytoplasmic staining for Rab25 and Rab35, and nuclear staining for AR. Breast, thyroid and prostate sections were used as positive controls for Rab25, Rab35 and AR, respectively.

Article Snippet: Monoclonal mouse anti-human Rab25 antibody was used at a concentration of 1 : 500 (clone 3F12F3, ProMab Biotechnologies, Richmond, CA, USA).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: British Journal of Cancer

Article Title: Androgen-related expression of G-proteins in ovarian cancer

doi: 10.1038/sj.bjc.6605153

Figure Lengend Snippet: Correlation of expression of Rab25 and Rab35 with clinical parameters

Article Snippet: Monoclonal mouse anti-human Rab25 antibody was used at a concentration of 1 : 500 (clone 3F12F3, ProMab Biotechnologies, Richmond, CA, USA).

Techniques: Expressing

Journal: British Journal of Cancer

Article Title: Androgen-related expression of G-proteins in ovarian cancer

doi: 10.1038/sj.bjc.6605153

Figure Lengend Snippet: High expression of Rab25 predicts survival by histological type. ( A ) Kaplan–Meier survival curves of all patients included in this study show differential expression by histological type. ( B ) Kaplan–Meier curves of only those tumours showing low expression of Rab25 shows no difference in survival ( C ) Kaplan–Meier curves of only those tumours showing high expression of Rab25 attenuates this finding with improved survival in mucinous and endometrioid tumours compared with serous and clear cell cancers ( P =0.043).

Article Snippet: Monoclonal mouse anti-human Rab25 antibody was used at a concentration of 1 : 500 (clone 3F12F3, ProMab Biotechnologies, Richmond, CA, USA).

Techniques: Expressing, Quantitative Proteomics

Journal: Communications Biology

Article Title: Analysis of the glyco-code in pancreatic ductal adenocarcinoma identifies glycan-mediated immune regulatory circuits

doi: 10.1038/s42003-021-02934-0

Figure Lengend Snippet: List of Antibodies used in this paper.

Article Snippet: Purified anti-human RAB25 , Cell Signaling , 13048 T , 1:500.

Techniques: Flow Cytometry, Microscopy, Western Blot, Purification, Enzyme-linked Immunosorbent Assay

Journal: Oncology Research

Article Title: Knockdown of Ras-Related Protein 25 (Rab25) Inhibits the In Vitro Cytotoxicity and In Vivo Antitumor Activity of Human Glioblastoma Multiforme Cells

doi: 10.3727/096504016X14736286083065

Figure Lengend Snippet: Knockdown of Rab25 inhibits EMT phenotype in GBM cells. U87MG cells were transfected with sh-Rab25 or sh-NC for 48 h. (A) Western blot analysis was used to evaluate the protein levels of E-cadherin, N-cadherin, and vimentin. (B) Statistical analysis of E-cadherin, N-cadherin, and vimentin levels in the sh-NC and sh-Rab25 groups. * p < 0.05.

Article Snippet: The antibodies against Rab25, E-cadherin, N-cadherin, vimentin, p-PI3K, PI3K, p-AKT, AKT, and GAPDH were from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Techniques: Knockdown, Transfection, Western Blot